Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig
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چکیده
منابع مشابه
Generation and analysis of expressed sequence tags (ESTs) from cambium tissue cDNA libraries of contrasting genotypes of Eucalyptus globulus Labill
In the present study, reported the generation and analysis of ESTs of cDNA libraries from cambium tissue of secondary xylem obtained of two genotypes of Eucalyptus globulus contrasting in wood density and pulp yield. The sequences were blasted and annotated to compare with genome and ESTs database of other plant species. The goal of study was to determinate wich genes was differentially express...
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In this study we successfully constructed a full-length cDNA library from Siberian tiger, Panthera tigris altaica, the most well-known wild Animal. Total RNA was extracted from cultured Siberian tiger fibroblasts in vitro. The titers of primary and amplified libraries were 1.30×10(6) pfu/ml and 1.62×10(9) pfu/ml respectively. The proportion of recombinants from unamplified library was 90.5% and...
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The number of sequences generated by genome projects has increased exponentially, but gene characterization has not followed at the same rate. Sequencing and analysis of full-length cDNAs is an important step in gene characterization that has been used nowadays by several research groups. In this work, we have selected Schistosoma mansoni clones for full-length sequencing, using an algorithm th...
متن کاملGeneration and analysis of expressed sequence tags from a cDNA library of the fruiting body of Ganoderma lucidum
BACKGROUND Little genomic or trancriptomic information on Ganoderma lucidum (Lingzhi) is known. This study aims to discover the transcripts involved in secondary metabolite biosynthesis and developmental regulation of G. lucidum using an expressed sequence tag (EST) library. METHODS A cDNA library was constructed from the G. lucidum fruiting body. Its high-quality ESTs were assembled into uni...
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Sago palm, or Metroxylon sagu, is a hardy and versatile plant that is able to tolerate many stresses, biotic and abiotic, during its growth. It is one of the plants that are able to grow in waterlogged area where others could not. Apart from that sago palm is also a source of starch, contributes economically to the people and an important export for the state of Sarawak. Despite the importance ...
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ژورنال
عنوان ژورنال: Journal of Integrative Agriculture
سال: 2014
ISSN: 2095-3119
DOI: 10.1016/s2095-3119(13)60414-1